Phosphatase inhibitors influence proteolytic cleavage pattern of glycine transporter GlyT2 N-terminus
نویسندگان
چکیده
Calpain activation in spinal cord neurons results in removal of the glycine transporter N-terminal region. In this work we have compared the cleavage patterns of released peptides in presence of both okadaic acid and sodium orthovanadate. Under basal calcium concentration the calpain cleavage patterns are significantly different for these two phosphatase inhibitors. In contrast to one immunopositive peptide band present in presence of sodium orthovanadate, two additional bands are observed in presence of okadaic acid. This indicates that phosphorylation might block/activate certain proteolytic cleavage sites on GlyT2 N-terminus in vivo.
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